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KMID : 0357920130470060541
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Korean Journal of Pathology
2013 Volume.47 No. 6 p.541 ~ p.548
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Immunohistochemical Classification of Primary and Secondary Glioblastomas
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Lee Kyu-Sang
Choe Ghee-Young
Nam Kyung-Han
Seo An-Na
Yun Su-Mi
Kim Kyung-Ju
Cho Hwa-Jin
Park Sung-Hye
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Abstract
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Background: Glioblastomas may develop de novo (primary glioblastomas, P-GBLs) or through progression from lower-grade astrocytomas (secondary glioblastomas, S-GBLs). The aim of this study was to compare the immunohistochemical classification of glioblastomas with clinically determined P-GBLs and S-GBLs to identify the best combination of antibodies for immunohistochemical classification.
Methods: We evaluated the immunohistochemical expression of epidermal growth factor receptor (EGFR), p53, and isocitrate dehydrogenase 1 (IDH-1) in 150 glioblastoma cases.
Results: According to clinical history, the glioblastomas analyzed in this study consisted of 146 P-GBLs and 4 S-GBLs. Immunohistochemical expression of EGFR, p53, and IDH-1 was observed in 62.6%, 49.3%, and 11.1%, respectively. Immunohistochemical profiles of EGFR(+)/ p53(?), IDH-1(?)/EGFR(+)/p53(?), and EGFR(?)/p53(+) were noted in 41.3%, 40.2%, and 28.7%, respectively. Expression of IDH-1 and EGFR(?)/p53(+) was positively correlated with young age. The typical immunohistochemical features of S-GBLs comprised IDH-1(+)/EGFR(?)/p53(+), and were noted in 3.6% of clinically P-GBLs. The combination of IDH-1(?) or EGFR(+) was the best set of immunohistochemical stains for identifying P-GBLs, whereas the combination of IDH-1(+) and EGFR(?) was best for identifying S-GBLs.
Conclusions: We recommend a combination of IDH-1 and EGFR for immunohistochemical classification of glioblastomas. We expect our results to be useful for determining treatment strategies for glioblastoma patients.
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KEYWORD
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Glioblastoma, Immunohistochemistry, IDH1 protein, human, Genes, erbB-1, Genes, p53
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